Peripheral blood stem cell collection from healthy donors for allogeneic transplantation

摘要: There is great interest in the use of peripheral blood stem cells (PBSC) for allogeneic transplantation, based on good results seen with autologous PBSC infusion. Reasonable caution exists regarding transplantation because donor toxicities due to rhG-CSF administration and risk graft-versus-host-disease (GVHD) recipient large number T-cell infused. We present preliminary data collections ten patients affected by advanced leukemia (eight patients), severe aplastic anemia (one patient) sickle cell patient). Seven donors were HLA-identical siblings, while other three mismatched three, two one locus, respectively. All received at a dose 12 micrograms/kg mean 5 days. Leukaphereses performed aim collecting minimum x 10(6)/kg (recipient's weight) CD 34+ cells. Collection timing was determined monitoring donor's from second day therapy. The yielded 10.05 10(8) MNCs/kg 10.48 10(6) cells/kg weight). immediately infused after collection given myeloablative Engraftment observed each patient 13.2 days an absolute neutrophil count (ANC) more than 0.5 10(9)/L 26.5 platelet 20 10(9)/L. Eight experienced no or moderate acute GVHD, whereas died grade 4 notwithstanding GVHD prophylaxis cyclosporine prednisone. Two viral fungal infections, respectively, despite prophylaxis. remaining six are alive between 58 430 transplant. Our document that capable engraftment regimen. Controlled trials necessary compare potential benefits this approach obtained bone marrow transplantation.