The petite mutation in yeasts: 50 years on.

摘要: Fifty years ago it was reported that baker's yeast, Saccharomyces cerevisiae, can form “petite colonie” mutants when treated with the DNA-targeting drug acriflavin. To mark jubilee of studies on cytoplasmic inheritance, a review early work will be presented together some observations current developments. The primary emphasis is to address questions how loss mtDNA leads lethality (ρ0-lethality) in petite-negative yeasts and S. cerevisiae tolerates elimination mtDNA. Recent investigations have revealed ρ0-lethality suppressed by specific mutations α, β, γ subunits mitochondrial F1-ATPase yeast Kluyveromyces lactis nuclear ptp alleles Schizosaccharomyces pombe. In contrast, inactivation genes coding for α β disruption AAC2, PGS1/PEL1, YME1 convert this petite-positive into form. Studies affecting dependence provided important insight functions genome maintenance structural functional integrity inner membrane.