Apoptosis in glioma cells: review and analysis of techniques used for study with focus on the laser scanning cytometer

摘要: Traditional approaches to the treatment of brain tumors are based on hypothesis that arise and grow because disordered regulation cell proliferation. More recently, it has become apparent tumor growth depends not only rate proliferation but also apoptosis (programmed death). Genomic alterations occur in malignancy may limit cell's ability undergo apoptosis. Many new strategies for gliomas stem from use techniques aimed at manipulating Being able assess efficacy experimental treatments with refined being instruments can provide accurate measurements apoptotic markers will open door discovering novel potential induce effective selective cytotoxicity. We discuss here detail major traditional assessing an overview cytometric techniques, including flow cytometry (FC), compare laser scanning cytometer (LSC). This is a powerful tool obtaining fast objective analysis through multiple mechanisms, as well DNA ploidy solid malignant tumors.