Methods of Determining Apoptosis in Neuro-Oncology

摘要: Tumor growth depends not only on the rate of cell proliferation but also programmed death (apoptosis). Thus, treatments focusing either restoration apoptosis or triggering apoptotic pathways may provide new treatment targets. Therefore, ability to detect in experimental studies is vital our assessment success. Apoptosis detection important assessing outcomes both and clinical settings. Furthermore, analysis surgically removed specimens for provides patient-specific data, allowing tailored plans. Many techniques are currently used apoptosis, including identifying those cells exhibiting pathognomonic morphological characteristics DNA breaks by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay, externalized phosphatidylserine, flow cytometry techniques, laser scanning cytometry, poly(ADP-ribose) polymerase cleavage, caspase activation, membrane permeability changes, mitochondrial failure, denatured DNA, as well a recent noninvasive technique measuring with novel fluorescence reporter. This chapter an overview these various methods emphasis glioma research.