Influence of chronic ethanol consumption on extra-pancreatic secretory function in rat.

摘要: BACKGROUND The usefulness of the typical direct methods involving duodenal intubation, such as secretin and secretin-cholecystokinin tests, in diagnosis exocrine pancreatic dysfunction is widely accepted. However, these diagnostic tests tend to be avoided because their technical complexity burden on patients. Recently, a simple breath test was developed for assessment function employing 13C-dipeptide [i.e., benzoyl-L-tyrosyl-[1-13C] alanine (Bz-Tyr-Ala)]. Although alcohol abuse causes damage humans, this has been unclear rats. AIMS aim study evaluate effect ethanol exposure beginning at an early age extra-pancreatic secretory MATERIALS AND METHODS Twelve female rats F344 strain aged 12 months were used. Seven fed commercial mash food with 16% solution (Japanese Sake) drinking-fluid since 29 days (ethanol group). remaining five nutrient-matched isocaloric diet water (control After 24-hr fasting, are orally administrated 1cc containing sodium (5 mg/kg) housed animal chamber. expired air chamber collected breath-sampling bag using tube aspiration pump. 13CO2 concentration measured infrared spectrometer 10-min interval 120 min expressed delta per mil. RESULTS level increased peaked 20 both two groups. In general, excretion rapidly also decreased sooner than control mean value maximal 34.7 mil rats, greater (31.4 mil), but difference did not reach statistically significance. CONCLUSION Chronic feeding does affect