The effect of chronic ethanol consumption on the fatty acid composition of phosphatidylinositol in rat liver microsomes as determined by gas chromatography and 1H-NMR.

摘要: Abstract Cell membranes and vesicles composed of extracted phospholipids isolated from rats chronically-fed ethanol develop a resistance to disordering by in vitro (membrane tolerance) decreased partitioning into the membranes. The anionic lipid phosphatidylinositol (PtdIns) is only microsomal phospholipid ethanol-fed that confers tolerance control paradigm where classes are sequentially swapped. To investigate molecular basis this adaptation, fatty acid content PtdIns livers chronically fed for 5 weeks their calorically-matched controls was analyzed gas-liquid chromatography (GLC) 1H-NMR spectroscopy. Chronic consumption caused an 8.4% decrease arachidonic [[20:4(n−6)], 20.0% increase oleic [18:1(n−9)] 47.1% quantitatively minor acid, eicosatrienoic [20:3(n−6)]. used assay compositional changes Δ5 olefinic moiety acyl chains PtdIns, approach should be broadly applicable other systems. After chronic feeding had unsaturates (−7.9% NMR, −8.2% GLC) corresponding saturates (+5.4% +5.3% GLC). In phospholipids, alterations compositions specific each phospholipid. PtdInd exhibited significant both polyunsaturate pool ratio total saturated content. major adaptative response rat liver administration involves [20:4(n−6)], which partly compensated increases iecosatrienoic [20:3(n−6)], resulting depressed unsaturation polyunsaturation index. at position may have special functional relevance, due tot he proximity membrane surface, believed reside. Whether these merely coincident with, or causative of, requires further elucidation.