TAC102 Is a Novel Component of the Mitochondrial Genome Segregation Machinery in Trypanosomes

摘要: Trypanosomes show an intriguing organization of their mitochondrial DNA into a catenated network, the kinetoplast (kDNA). While more than 30 proteins involved in kDNA replication have been described, only few components segregation machinery are currently known. Electron microscopy studies identified high-order structure, tripartite attachment complex (TAC), linking basal body flagellum via membranes to kDNA. Here we describe TAC102, novel core component TAC, which is essential for proper during cell division. Loss TAC102 leads genome missegregation but has no impact on organelle biogenesis and segregation. The protein present throughout cycle assembled newly developing TAC after pro-basal matured indicating hierarchy assembly process. Furthermore, provide evidence that replicated de novo rather using semi-conservative mechanism. Lastly, demonstrate lacks N-terminal targeting sequence requires sequences C-terminal part its localization.