Multiplex Fluorogenic Real-Time PCR for Detection and Quantification of Escherichia coli O157:H7 in Dairy Wastewater Wetlands

作者: A. Mark Ibekwe , Pamela M. Watt , Catherine M. Grieve , Vijay K. Sharma , Steven R. Lyons

DOI: 10.1128/AEM.68.10.4853-4862.2002

关键词:

摘要: … O157:H7 levels detected in wetland samples decreased by about 2 logs between wetland influents and effluents. The detection … coli O157:H7 in environmental samples and represents …

参考文章(32)
PINA M. FRATAMICO, LORI K. BAGI, TIZIANA PEPE, A multiplex polymerase chain reaction assay for rapid detection and identification of Escherichia coli O157:H7 in foods and bovine feces. Journal of Food Protection. ,vol. 63, pp. 1032- 1037 ,(2000) , 10.4315/0362-028X-63.8.1032
R. D. Oberst, M. P. Hays, L. K. Bohra, R. K. Phebus, C. T. Yamashiro, C. Paszko-Kolva, S. J. A. Flood, J. M. Sargeant, J. R. Gillespie, PCR-based DNA amplification and presumptive detection of Escherichia coli O157:H7 with an internal fluorogenic probe and the 5' nuclease (TaqMan) assay Applied and Environmental Microbiology. ,vol. 64, pp. 3389- 3396 ,(1998) , 10.1128/AEM.64.9.3389-3396.1998
M Louie, J C de Azavedo, M Y Handelsman, C G Clark, B Ally, M Dytoc, P Sherman, J Brunton, Expression and characterization of the eaeA gene product of Escherichia coli serotype O157:H7. Infection and Immunity. ,vol. 61, pp. 4085- 4092 ,(1993) , 10.1128/IAI.61.10.4085-4092.1993
Vernon L. Tesh, Alison D. O'Brien, Adherence and colonization mechanisms of enteropathogenic and enterohemorrhagic Escherichia coli Microbial Pathogenesis. ,vol. 12, pp. 245- 254 ,(1992) , 10.1016/0882-4010(92)90043-N
Claudio Orlando, Pamela Pinzani, Mario Pazzagli, Developments in quantitative PCR Clinical Chemistry and Laboratory Medicine. ,vol. 36, pp. 255- 269 ,(1998) , 10.1515/CCLM.1998.045
Vijay K. Sharma, Steve A. Carlson, Simultaneous Detection of Salmonella Strains and Escherichia coli O157:H7 with Fluorogenic PCR and Single-Enrichment-Broth Culture Applied and Environmental Microbiology. ,vol. 66, pp. 5472- 5476 ,(2000) , 10.1128/AEM.66.12.5472-5476.2000
Wolfgang Ludwig, Karl-Heinz Schleifer, How quantitative is quantitative PCR with respect to cell counts Systematic and Applied Microbiology. ,vol. 23, pp. 556- 562 ,(2000) , 10.1016/S0723-2020(00)80030-2