Fluctuations of T- and B-cell subsets in basic protein-induced experimental allergic encephalomyelitis (EAE) in long-tailed macaques

摘要: Experimental allergic encephalomyelitis (EAE) was induced in long-tailed macaques (Macaca fascicularis) by inoculation of autologous myelin basic protein (BP) complete Freund's adjuvant. Natural killer (NK) cell activity and lymphocyte subsets detected one- two-color immunofluorescence were monitored longitudinally these animals. A decrease NK at the onset clinically defined disease. During preclinical phase EAE (5-7 days before clinical signs) absolute number T helper (CD4+) suppressor (CD8+) cells peripheral blood decreased significantly. Analysis B revealed a selective depletion IgD+ corresponding increase IgD- prior to during signs. Total B-cell numbers not significantly different between normal groups. The increased proportion BP-sensitized animals corresponded with appearance high titers circulating anti-BP antibodies. Thus analysis may be sensitive indicator activation abnormal immune status EAE. Changes are compared those humans multiple sclerosis.