作者: R. Dontula , A. Dinasarapu , C. Chetty , P. Pannuru , E. Herbert
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摘要: Glioblastoma (GBM) is the most common and malignant primary adult brain cancer. Allelic deletion on chromosome 14q plays an important role in pathogenesis of GBM, this site was thought to harbor multiple tumor suppressor genes associated with a region that also encodes microRNA-203 (miR-203). In study, we sought identify miR-203 as GBM. We analyzed expression data GBM patients 10 normal 495 tissue samples derived from The Cancer Genome Atlas set. Quantitative realtime PCR situ hybridization high-grade low-grade anaplastic astrocytoma showed decreased levels tissues cell lines. Exogenous using plasmid expressing precursor (pmiR-203) suppressed glioma proliferation, migration, invasion. determined one relevant target Robo1, given mRNA protein by RT-PCR Western blot analysis. Moreover, cotransfection experiments luciferase-based transcription reporter assay have shown direct regulation Robo1 miR-203. show mediates mediated antimigratory functions up-regulation abrogates effects. ERK phosphorylation MMP-9 cells. Furthermore, demonstrate inhibits migration cells disrupting Robo1/ERK/MMP-9 signaling axis. Taken together, these studies response decrease responsible for