Visualization of Nanofibrillar Cellulose in Biological Tissues Using a Biotinylated Carbohydrate Binding Module of β-1,4-Glycanase.

摘要: Nanofibrillar cellulose is a very promising innovation with diverse potential applications including high quality paper, coatings, and drug delivery carriers. The production of nanofibrillar on an industrial scale may lead to increased exposure both in the working environment general environment. Assessment health effects following therefore required. However, as primarily consists glucose moieties, detection biological tissues difficult. We have developed simple robust method for specific sensitive fibers, cellulose, tissue, using biotinylated carbohydrate binding module (CBM) β-1,4-glycanase (EXG:CBM) from bacterium Cellulomonas fimi. EXG:CBM was expressed Eschericia coli, purified, biotinylated. shown bind quantitatively five different cellu...