A Genetic Porcine Model of Cancer
作者： Lawrence B Schook , Tiago V Collares , Wenping Hu , Ying Liang , Fernanda M Rodrigues
DOI: 10.1371/JOURNAL.PONE.0128864
关键词:
摘要: The large size of the pig and its similarity in anatomy, physiology, metabolism, genetics to humans make it an ideal platform develop a genetically defined, animal model cancer. To this end, we created transgenic “oncopig” line encoding Cre recombinase inducible porcine transgenes KRASG12D TP53R167H, which represent commonly mutated oncogene tumor suppressor human cancers, respectively. Treatment cells derived from these oncopigs with adenovirus (AdCre) led TP53R167H expression, rendered transformed culture tumorigenic when engrafted into immunocompromised mice. Finally, injection AdCre directly rapid reproducible development mesenchymal origin. Transgenic animals receiving AdGFP (green fluorescent protein) did not have any mass formation or altered histopathology. This oncopig could thus serve as malleable for potentially wide spectrum while controlling temporal spatial genesis, should prove invaluable studies previously hampered by lack
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K. N. Kuzmuk, L. B. Schook, Pigs as a model for biomedical sciences. CABI Publishing. pp. 426- 444 ,(2011) , 10.1079/9781845937560.0426
Malachi Griffith, Obi L. Griffith, COSMIC (Catalogue of Somatic Mutations in Cancer) Dictionary of Bioinformatics and Computational Biology. ,(2004) , 10.1002/9780471650126.DOB0851
L.B. Schook, K. Kuzmuk, S. Adam, L. Rund, K. Chen, M. Rogatcheva, M. Mazur, C. Pollock, C. Counter, DNA-based Animal Models of Human Disease: from Genotype to Phenotype Developments in biologicals. ,vol. 132, pp. 15- 25 ,(2008) , 10.1159/000317140
The genetics of the pig The genetics of the pig.. ,(2011) , 10.1079/9781845937560.0000
Yuliya Pylayeva-Gupta, Elda Grabocka, Dafna Bar-Sagi, RAS oncogenes: weaving a tumorigenic web Nature Reviews Cancer. ,vol. 11, pp. 761- 774 ,(2011) , 10.1038/NRC3106
Callie B. Pollock, Margarita B. Rogatcheva, Lawrence B. Schook, Comparative genomics of xenobiotic metabolism: a porcine-human PXR gene comparison. Mammalian Genome. ,vol. 18, pp. 210- 219 ,(2007) , 10.1007/S00335-007-9007-7
Sunil R. Hingorani, Lifu Wang, Asha S. Multani, Chelsea Combs, Therese B. Deramaudt, Ralph H. Hruban, Anil K. Rustgi, Sandy Chang, David A. Tuveson, Trp53R172H and KrasG12D cooperate to promote chromosomal instability and widely metastatic pancreatic ductal adenocarcinoma in mice Cancer Cell. ,vol. 7, pp. 469- 483 ,(2005) , 10.1016/J.CCR.2005.04.023
Erica L Jackson, Nicholas Willis, Kim Mercer, Roderick T Bronson, Denise Crowley, Raymond Montoya, Tyler Jacks, David A Tuveson, Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras Genes & Development. ,vol. 15, pp. 3243- 3248 ,(2001) , 10.1101/GAD.943001
Maurice J.B. van den Hoff, Antoon F.M. Moorman, Wouter H. Lamers, Electroporation in ‘intracellular’ buffer increases cell survival Nucleic Acids Research. ,vol. 20, pp. 2902- 2902 ,(1992) , 10.1093/NAR/20.11.2902
R. C. O'Hagan, J. Heyer, KRAS Mouse Models Modeling Cancer Harboring KRAS Mutations Genes & Cancer. ,vol. 2, pp. 335- 343 ,(2011) , 10.1177/1947601911408080