Studies of baker's yeast metabolism. II. The role of adenine nucleotides and inorganic phosphate in the control of respiration during alcohol oxidation.

摘要: Abstract The respiratory inhibition following within 40 seconds after the addition of ethanol to a starved baker's yeast cell suspension has been studied by analysis intracellular adenine nucleotides and phosphate. Experiments with chemicals like glucose, 2-deoxyglucose, or glucose in presence iodoacetic acid have revealed that observed is caused unavailability adenosine 5′-diphosphate. By use suitable phosphate trap, such as 2-deoxyglucose it possible demonstrate alcohol-oxidizing mediated lack inorganic A true Crabtree effect brought forth could not be demonstrated, although net rate shown acid. data discussed relation general problem control metabolism intact systems.