Characterisation of Mycobacterium tuberculosis isolates lacking IS6110 in Viet Nam

摘要: SETTING: The molecular diagnosis of tuberculosis (TB) in Viet Nam is often based on the detection insertion sequence (IS) 6110 Mycobacterium tuberculosis. However, 8-11% M. strains South-East Asia do not contain this target and undermines validity these tests. OBJECTIVE: We quantified frequency lacking IS6110 rural studied their epidemiological clinical characteristics. DESIGN: Consecutively diagnosed adult TB patients Southern submitted two sputum samples for culture, restriction fragment length polymorphism (RFLP) spoligotyping 15-loci variable number tandem repeat typing. Polymerase chain reaction (PCR) was performed to confirm absence elements hybridisation RFLP. RESULTS: Among 2664 patient isolates examined, 109 (4.1%) had no element. Compared other strains, no-copy were less resistant anti-tuberculosis drugs, particularly streptomycin (adjusted OR 0.2, 95%CI 0.1-0.5), showed significant geographic variation. No associations with history or demographic factors found. CONCLUSIONS: Strains without pose a problem as regards false-negative PCR. circulating Nam, are more susceptible drugs.