Preparation and characterization of bone marrow mesenchymal stem cell-derived extracellular matrix scaffolds.
作者： Yan Xu , Guang‐yue Xu , Cheng Tang , Bo Wei , Xuan Pei
DOI: 10.1002/JBM.B.33231
关键词:
摘要: The aim of this study was to assess the feasibility creating extracellular matrix (ECM) scaffolds from mesenchymal stem cells. Bone marrow cell (BMSC)-derived ECM (BMSC-dECM) were fabricated by lyophilization after crosslinking, without using a decellularization process. Acellular porcine chondrocyte-derived (AC-dECM) used as control. surface morphology, internal structure, water uptake ratio, mechanical properties, and biocompatibility scaffolds, well in vitro behavior cells grown on examined compared between two scaffold types. For BMSC-dECM average pore size 304.4 ± 108.2 μm, porosity 93.3% ± 4.5%, compressive modulus 6.8 ± 1.5 kPa, ratio exceeded 20. supported attachment proliferation cells, with these aspects likely being comparable those AC-dECM scaffolds. findings preliminary highlight potential utility BMSC-derived for future cartilage tissue-engineering applications. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 670–678, 2015.
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