In vivo cartilage tissue engineering using a cell-derived extracellular matrix scaffold.

摘要: We have observed in our previous study that a cell-derived extracellular matrix (ECM) scaffold could assure the growth of cartilage tissue construct vitro. The purpose present was to evaluate feasibility chondrocyte-seeded ECM by implanting it vivo nude mouse. A porous prepared with freeze-drying protocol using porcine chondrocytes. Rabbit articular chondrocytes were seeded onto and cultured for 2 days vitro, then implanted into mouse subcutaneously. They retrieved at 1, 2, 3 weeks postimplantation. Under macroscopic analysis, cartilage-like formation matured time developed smooth, white surface. Contrary control (in which no cells seeded), size neocartilage increased slightly third week remained more stable. Total glycosaminoglycan (GAG) content GAG/DNA ratio significantly chemical analysis. histology exhibited sustained accumulation newly synthesized sulfated proteoglycans. Immunohistochemistry, Western blot, reverse transcriptase-polymerase chain reaction (RT-PCR) clearly identified type II collagen all points. Compressive strength from 0.45 +/- 0.06 MPa 1 1.18 0.17 weeks. In conclusion, this demonstrated provide favorable environment produce hyaline-like tissue.