Δ133p53α, a natural p53 isoform, contributes to conditional reprogramming and long-term proliferation of primary epithelial cells.

摘要: We previously developed the technique of conditional reprogramming (CR), which allows primary epithelial cells from fresh or cryopreserved specimens to be propagated long-term in vitro, while maintaining their genetic stability and differentiation potential. This method requires a combination irradiated fibroblast feeder Rho-associated kinase (ROCK) inhibitor. In present study, we demonstrate increased levels full-length p53 its natural isoform, Δ133p53α, conditionally reprogrammed prostate, foreskin, ectocervical, mammary tissues. Increased Δ133p53α expression is critical for CR since cell proliferation rapidly inhibited following siRNA knockdown endogenous Δ133p53α. Importantly, overexpression consistently delays onset cellular senescence when cultured under non-CR conditions normal keratinocyte growth medium (KGM). More significantly, ROCK inhibitor, without cells, enables vitro. also show that induces hTERT telomerase activity causes rapid inhibition proliferation, indicating role mediating effects Altogether, these data functional regulatory link between pathways during cells.