A dynamic system for single and repeated exposure of airway epithelial cells to gaseous pollutants.

摘要: In vitro models are promising approaches to investigate the adverse effects and mode of action air pollutants on respiratory tract. We designed a dynamic system that allows single or repeated exposure cultured cells two major indoor gaseous pollutants, formaldehyde (HCHO) nitrogen dioxide (NO2), alone as mixture. this system, Calu-3 human bronchial epithelial cell line was exposed at air–liquid interface (ALI) submerged by culture medium synthetic target concentrations HCHO and/or NO2 once 4 consecutive days before assessment viability necrosis, IL-6 IL-8 release trans-epithelial electrical resistance. Our data showed whereas ALI method can be used for short-term exposures only, provides possibility expose in manner. As well, we found indoors triggered significant decrease metabolism an increase were not evoked exposure. Thus, our work highlights fact development systems methods allow compounds mixtures is interest evaluate impact pollution