Mutations potentially associated with decreased susceptibility to fluoroquinolones, macrolides and lincomycin in Mycoplasma synoviae.

摘要: Mycoplasma synoviae is one of the economically most significant avian species. It can cause great financial losses to poultry industry by inducing respiratory diseases, infectious synovitis, or eggshell apex abnormalities. There are different approaches control M. infection. Although antimicrobial therapy cannot replace long-term solutions, like eradication and vaccination, this strategy be effective in short term, as adequate antibiotic treatment relieve economic through attenuation clinical signs reduction transmission. Using broth microdilution method, minimal inhibitory concentration (MIC) values fourteen antibiotics related eight groups were determined 96 strains. Whole genome sequencing sequence analysis revealed mutations potentially associated with decreased susceptibility fluoroquinolones, macrolides lincomycin. Molecular markers responsible for high MICs fluoroquinolones found gyrA, gyrB, parC parE genes. Besides, single nucleotide polymorphisms identified genes encoding 23S rRNA 50S inhibitor lincomycin, while amino acid change ribosomal protein L22 could macrolides. The contribute extension knowledge about genetic background resistance synoviae. Moreover, explored resistance-related may serve targets molecular biological assays providing data prior laborious time-consuming isolation