Zebrafish cDNA Encoding Multifunctional Fatty Acid Elongase Involved in Production of Eicosapentaenoic (20:5n-3) and Docosahexaenoic (22:6n-3) Acids
作者： Morris Agaba , DouglasR. Tocher , CathrynA. Dickson , JamesR. Dick , AlanJ. Teale
DOI: 10.1007/S10126-003-0029-1
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摘要: Enzymes that increase the chain length of fatty acids are essential for biosynthesis highly unsaturated acids. The gLELO gene encodes a protein involved in elongation polyunsaturated fungus Mortierella alpina. A search GenBank database identified several expressed sequence tag sequences, including one obtained from zebrafish (Danio rerio), with high similarity to gLELO. full-length transcript ZfELO, encoding polypeptide 291 amino acid residues, was isolated liver cDNA. predicted open reading frame shared elongases Caenorhabditis elegans and human. When Saccharomyces cerevisiae, conferred ability lengthen range C18, C20, C22 acids, indicating not only 22:6n-3 18:3n-3 via 24-carbon intermediate is feasible, but also elongase enzyme can perform all three steps required. able elongate monounsaturated saturated thus demonstrates greater level promiscuity terms substrate use than any described previously.
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