Phleomycin increases transformation efficiency and promotes single integrations in Schizophyllum commune.

摘要: Phleomycin is mutagenic by introducing double-strand breaks in DNA. The ble gene of Streptoalloteychus hindustanus, which confers resistance to this substance, widely used as a selection marker for transformation. Schizophyllum commune grows on 25 μg phleomycin ml−1 after introduction cassette based the gene. However, we here report that growth resistant colonies concentration resulted aberrant colony morphologies. Apparently, was despite acquired resistance. Therefore, new system developed antibiotic nourseothricin. transformation efficiency tenfold lower than obtained with agent. This low could be rescued addition nonselective during protoplast regeneration. accompanied higher incidence single-copy integrations and an increase expression key genes involved break repair. Taken together, conclude effect strongly resembles restriction enzyme-mediated integration (REMI) but, unlike REMI, it does not depend presence target site.