Modification of the plasma complement protein profile by exogenous estrogens is indicative of a compromised immune competence in marine medaka (Oryzias melastigma).

摘要: Growing evidence suggests that the immune system of teleost is vulnerable to xenoestrogens, which are ubiquitous in marine environment. This study detected and identified major circulatory proteins deregulated by 17α-ethinylestradiol (EE2), may be linked fish susceptibility pathogens medaka, Oryzias melastigma. Fish competence was determined using a host resistance assay pathogenic bacteria Edwardsiella tarda. Females were consistently more susceptible infection-induced mortality than males. Exposure EE2 could narrow sex gap increasing death male fish. Proteomic analysis revealed plasma adult highly sexually dimorphic. induced pronounced sex-specific changes proteome, with composition clearly becoming "feminised". Male found contain higher level fibrinogens, WAP63 ependymin-2-like protein, involved coagulation, inflammation regeneration. For first time, we demonstrated expression C1q subunit B (C1Q), an initiating factor classical complement pathway, males suppressed both sexes response bacterial challenge. Moreover, cleavage post-translational modification C3, central component system, altered treatment (C3dg down; C3g up). Multiple regression indicated C1Q possibly indicator survival, warrants further confirmation. The findings support potential application for prognosis/diagnosis competence. this provides biochemical basis sex-differences immunity how these differences might modified xenoestrogens.