The Changing Tails of a Novel Short Interspersed Element in Aedes aegypti: Genomic Evidence for Slippage Retrotransposition and the Relationship Between 3′ Tandem Repeats and the poly(dA) Tail

摘要: A novel family of tRNA-related SINEs named gecko was discovered in the yellow fever mosquito, Aedes aegypti. Approximately 7200 copies were distributed A. aegypti genome with a significant bias toward + T-rich regions. The 3′ end is similar sequence and identical secondary structure to MosquI, non-LTR retrotransposon Nine conserved substitutions deletion separate into two groups. Group I includes all that poly(dA) copy ends AGAT repeats. II comprises elements CCAA or CAAT Members within each group cannot be differentiated when repeats are excluded phylogenetic analyses, suggesting alterations tails recent. Imperfect tail recorded partial replication tandem frequently observed II. Genomic evidence underscores importance slippage retrotransposition alteration expansion repeat during evolution sequences, although we do not rule out postinsertion mechanisms previously invoked explain Alu-associated microsatellites. We propose may generated by both retrotransposons thus distinction between such as L1 non-poly(dA) factor informative.