A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata.
作者： Jack D. Sunter , Sonal P. Patel , Robert A. Skilton , Naftaly Githaka , Donald P. Knowles
DOI: 10.1016/J.GENE.2008.01.026
关键词: Tick 、 Ixodes scapularis 、 Ixodidae 、 Biology 、 Rhipicephalus 、 Amblyomma variegatum 、 Genome 、 Retroposon 、 genomic DNA 、 Genetics
摘要: Reassociation kinetics and flow cytometry data indicate that ixodid tick genomes are large, relative to most arthropods, containing ≥10 9 base pairs. The molecular basis for this is unknown. We have identified a novel small interspersed element with features of tRNA-derived SINE, designated Ruka, in genomic sequences Rhipicephalus appendiculatus Boophilus (Rhipicephalus) microplus ticks. SINE was also expressed sequence tag (EST) databases derived from several tissues four species ticks, namely R. appendiculatus, B. (R.) microplus, Amblyomma variegatum the more distantly related Ixodes scapularis. Secondary structure predictions indicated Ruka could adopt tRNA was, atypically, similar serine tRNA. By extrapolation frequency occurrence randomly selected BAC clone consistent approximately 65,000 copies genome. Real time PCR analyses on DNA copy numbers specific subsets between 5800 38,000. Several putative conserved insertion sites were EST three based flanking associated SINEs, indicating some transpositions probably occurred prior speciation within metastriate division Ixodidae. strongly suggest Class I transposable elements form significant component may partially account large genome sizes observed. © 2008 Elsevier B.V. All rights reserved.
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