The in vitro cultivation of P. falciparum ookinetes, and their enrichment on Nycodenz® density gradients

作者: E. H. Carter , A. Suhrbier , P. J. A. Beckers , R. E. Sinden

DOI: 10.1017/S0031182000057516

关键词:

摘要: Employing a simple method of growing ookinetes Plasmodium falciparum in culture, 40% mature gametocytes convert to macrogametes, 4% reach the retort-form ookinete stage and 0.45% become ookinetes. A single-step gradient centrifugation on 12.5% Nycodenz enriches both

参考文章(12)
Richard Carter, Robert W. Gwadz, Florence M. McAuliffe, Plasmodium gallinaceum: Transmission-blocking immunity in chickens Experimental Parasitology. ,vol. 47, pp. 185- 193 ,(1979) , 10.1016/0014-4894(79)90072-9
T. Ponnudurai, A.H.W. Lensen, Anna D.E.M. Leeuwenberg, J.H.E.Th. Meuwissen, Cultivation of fertile Plasmodium falciparum gametocytes in semi-automated systems. 1. Static cultures. Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 76, pp. 812- 818 ,(1982) , 10.1016/0035-9203(82)90116-X
C. J. Janse, B. Mons, R. J. Rouwenhorst, P. F. J. van der Klooster, J. P. Overdulve, H. J. van der Kaay, In vitro formation of ookinetes and functional maturity of Plasmodium berghei gametocytes. Parasitology. ,vol. 91, pp. 19- 29 ,(1985) , 10.1017/S0031182000056481
A. Knight, R.E. Sinden, The purification of gametocytes of Plasmodium falciparum and P. yoelii nigeriensis by colloidal silica (Percoll) gradient centrifugation Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 76, pp. 503- 509 ,(1982) , 10.1016/0035-9203(82)90150-X
Frank Hawking, Margaret E. Wilson, Kenneth Gammage, Evidence for cyclic development and short-lived maturity in the gametocytes of Plasmodium falciparum. Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 65, pp. 549- 559 ,(1971) , 10.1016/0035-9203(71)90036-8
MASAMICHI AIKAWA, RICHARD CARTER, YOSHIHIRO ITO, MARY M. NIJHOUT, New observations on gametogenesis, fertilization, and zygote transformation in Plasmodium gallinaceum. Journal of Eukaryotic Microbiology. ,vol. 31, pp. 403- 413 ,(1984) , 10.1111/J.1550-7408.1984.TB02987.X
Arno N. Vermeulen, Jan van Deursen, Ruud H. Brakenhoff, Ton H.W. Lensen, Thivi Ponnudurai, Joseph H.E.Th. Meuwissen, Characterization of Plasmodium falciparum sexual stage antigens and their biosynthesis in synchronised gametocyte cultures Molecular and Biochemical Parasitology. ,vol. 20, pp. 155- 163 ,(1986) , 10.1016/0166-6851(86)90027-7
Nirbhay Kumar, Richard Carter, Biosynthesis of the target antigens of antibodies blocking transmission of Plasmodium falciparum. Molecular and Biochemical Parasitology. ,vol. 13, pp. 333- 342 ,(1984) , 10.1016/0166-6851(84)90124-5