Isolation of O$ 3 $-Response Genes from Arabidopsis thaliana Using cDNA Macroarray

摘要: Nylon membrane-based cDNA macroarrays are a widely available alternative to microarrays for the collection of large-scale gene expression data. used in many areas molecular biology research applications ranging from discovery profiling. Although degree location DNA spot macroarray is lower than that microarray, it can be detect large number genes because uses radiolabeled as probe. Thus, technology applied obtain profile organs show wide variety mRNA expression, such meristems plant species and brain tissue. To carry out hybridization experiments with macroarray, I describe here how prepare filters on small or scale, well analyze determine statistical significance data obtained.