Differential ozone sensitivity among-Arabidopsis accessions and its relevance to ethylene synthesis

摘要: We compared the physiological and molecular responses of two Arabidopsis accessions, Col-0 Ws-2, to ozone (O3) exposure. Observation visible injury as well ion-leakage analysis demonstrated clear differences between O3-tolerant accession Col O3-sensitive Ws. RNA-blot showed that O3-induced increases in mRNA levels several ethylene-inducible genes a salicylic acid-inducible gene were substantially higher Ws than Col. The time-course induction various shows expression was rapidly, more strongly, induced by O3 Col, suggesting exhibits ethylene-signaling. Both level for an O3-inducible 1-aminocyclopropane-1-carboxylate synthase ethylene generation after 3 h O3-exposure leaf damage attenuated pretreatment with biosynthesis- signaling-inhibitors, indicating signaling is required On other hand, ethylene-overproducing mutant eto1-1, displayed significantly increased wild type plants. These results indicate difference sensitivity dependent on production rate these accessions. Finally, we investigated relationship degree evolution 20 different Based result, accessions classified into four types. However, most them significant correlation injury, important factor natural variety among