CD49f Is an Efficient Marker of Monolayer- and Spheroid Colony-Forming Cells of the Benign and Malignant Human Prostate

摘要: Stem cells may play a role in the development and maintenance of proliferative diseases prostate such as cancer benign prostatic hyperplasia. Cell membrane protein markers, CD49f, CD133 CD44, have been shown to identify putative stem cells, but lack consensus exists with regards most efficient marker(s) for stem-like cell identification. This study aimed determine whether previously reported markers had equal capacity select monolayer spheroid colony-forming (CFCs), which were used surrogate readouts characterize expression CD44 by flow cytometry immunohistochemistry. In CD49f+, CD44+, CD133+ represented 5.6±3.1%, 28.2±4.1% 0.10±0.06% total cells. Both monolayer- spheroid-CFCs existed at frequency approximately 0.5% subpopulations differed significantly their ability CFCs. The highest recovery CFCs was achieved CD49f+ selection (98%), whereas CD44+ or led poor CFC-recovery (17% 3%, respectively). For first time, we show highly from advanced not selection. Furthermore, (AC133 clone) could be detected either immunohistochemistry cytometry. We conclude that described optimal malignant prostate.