Real-time PCR for the detection of Salmonella spp. in food: An alternative approach to a conventional PCR system suggested by the FOOD-PCR project.

摘要: Abstract A real-time PCR assay using non-patented primers and a TaqMan® probe for the detection quantification of Salmonella spp. is presented. The based on an internationally validated conventional system, which was suggested as standard method in FOOD-PCR project. sensitive specific. Consistent 9.5 genome equivalents per reaction achieved, whereas samples containing average 0.95 were inconsistently positive. performed equally well commercially available allowed artificially contaminated food. After enrichment 16 h buffered peptone water (BPW) or universal pre-enrichment broth (UPB) 2.5 CFU/25 g salmon minced meat, 5 CFU/25 g chicken meat 25 ml raw milk detected. Enrichment BPW yielded higher numbers CFU/ml than UPB all matrices tested. However, productivity sufficient, positive with both methods, including those less 300 CFU/ml (enrichment 5 CFU/25 ml UPB).