Thymus-Repopulating Capacity of Cells that Can be Induced to Differentiate to T Cells in Vitro

摘要: It was established previously that committed precursors of T cells, which reside in bone marrow and spleen lack cell surface differentiation antigens, can be induced by thymopoietin certain other agents to differentiate rapidly vitro into cells bearing typical including Thy-1 TL (Komuro-Boyse assay). To relate this differentiative step observed physiologic events vivo, a system devised trace the migration precursor thymus, their maturation cells. Lethally irradiated mice - strain received from + hybrids i.v., population thymus enumerated 13 20 days later. Donor first became detectable at increased thereafter. Preliminary tests showed capable migrating have similar density are inducible Komuro-Boyse assay, being lower than mature The thymus-repopulating properties donor were not affected by: 1) pre-treatment with extract or thymopoietin, initiates precursors, nor b) anti serum plus complement, eliminates differentiated But a) applied sequence markedly reduced capacity repopulate thymus. These results interpreted as follows: induction (pro-thymocyte) yields (early thymocytes) yet lost property repopulating thymus; therefore, thymus-repopulation depleted treatment alone, did affect although treatments eliminate newly thus impaired repopulation We conclude responds assay expressing phenotype is same normally migrates vivo hemopoietic tissues there express an early cell.