Expression levels and functional aspects of the hyaluronan receptor CD44: Effects of insulin, glucose, IGF-I, or growth hormone on human arterial smooth muscle cells

摘要: An increased amount of hyaluronan (HA) in the arterial wall is a feature diabetic macroangiopathy. The functional consequences accumulated HA are mediated through binding to CD44. regulation this receptor by metabolic and hormonal factors is, however unknown. objective study was examine influence glucose, insulin, insulin-like growth factor I (IGF-I), human hormone (hGH) on formation function CD44 cultures aortic smooth muscle cells (SMCs). Migration nonproliferating SMCs were determined estimating area covered 6 days after removal barrier. Cellular content standard its isoforms, CD44v3 CD44v6, HA-binding capacity measured using modified enzyme-linked immunosorbent assay procedure. analysis made either with antibodies against or as ligand. migration showed that IGF-I able stimulate SMC (2P < .01). Anti-CD44 antibody inhibited stimulated at most concentrations. Insulin 100 1000 μU/mL insulin .03). expression only elevated .03), whereas decreased 2 ng/mL hGH 16 Glucose reduced variant isoform .01) but did not change total CD44v6 present SMCs. In conclusion, data obtained vitro support role isoform, CD44v3, response disorders diabetes.