A small-scale method for quantitation of carotenoids in bacteria and yeasts

摘要: Abstract Microbial carotenoids are difficult to extract because of their embedding into a compact matrix and prominent sensitivity degradation. Especially for carotenoid analysis bacteria yeasts, there is lack information about capability, precision recovery the method used. Accordingly, we investigated feasibility, throughput validity new small-scale using Micrococcus luteus Rhodotorula glutinis testing purposes. For disintegration extraction, combined primarily mild techniques: enzymatically used combinations lysozyme lipase as well lyticase yeasts. Additional mechanical treatment included sonication freeze-thawing cycles. Chemical with dimethylsulfoxide was applied yeasts only. extraction methanol-chloroform mixture stabilized efficiently butylated hydroxytoluene alpha-tocopherol. Separation compounds achieved HPLC, applying binary methanol/ tert -butyl methyl ether gradient on polymer reversed C30 phase. Substances interest were detected identified photodiode-array (PDA) quantitated all-trans-β-carotene equivalents. evaluation reproducibility method, β-8′-apo-carotenal internal standard. The provides sensitive tool determination from also small changes in spectrum single species. Corequisite large experiments facilitated by high method.