摘要: The epidermal growth factor (EGF) receptor interacts with plasma membrane-associated adapter proteins during endocytosis through coated pits. Almost 50 percent of the total pool alpha-adaptins was coimmunoprecipitated EGF when A-431 cells were treated at 37 degrees C, but not 4 C. Partial proteolysis alpha-adaptin suggested that amino-terminal domain is region associates receptor. extent receptor-adaptin association increased in depleted potassium to block endocytosis. These data suggest occurs intact before pits are fully assembled.
sciencemag.org
unirioja.es
harvard.edu参考文章(22)
J.P. Beltzer, M. Spiess, In vitro binding of the asialoglycoprotein receptor to the beta adaptin of plasma membrane coated vesicles. The EMBO Journal. ,vol. 10, pp. 3735- 3742 ,(1991) , 10.1002/J.1460-2075.1991.TB04942.X
Nobuyuki Kamata, Toshio Kuroki, Masaru Horikoshi, Kazuhiro Chida, Shoji Enomoto, Koichi Rikimaru, Growth-inhibitory Effects of Epidermal Growth Factor and Overexpression of Its Receptors on Human Squamous Cell Carcinomas in Culture Cancer Research. ,vol. 46, pp. 1648- 1653 ,(1986)
A. Sorkin, C. Waters, K.A. Overholser, G. Carpenter, Multiple autophosphorylation site mutations of the epidermal growth factor receptor. Analysis of kinase activity and endocytosis. Journal of Biological Chemistry. ,vol. 266, pp. 8355- 8362 ,(1991) , 10.1016/S0021-9258(18)92983-2
A Sorkin, K Helin, C.M. Waters, G Carpenter, L Beguinot, Multiple autophosphorylation sites of the epidermal growth factor receptor are essential for receptor kinase activity and internalization. Contrasting significance of tyrosine 992 in the native and truncated receptors. Journal of Biological Chemistry. ,vol. 267, pp. 8672- 8678 ,(1992) , 10.1016/S0021-9258(18)42495-7
A. Sorkin, G. Carpenter, Dimerization of internalized epidermal growth factor receptors. Journal of Biological Chemistry. ,vol. 266, pp. 23453- 23460 ,(1991) , 10.1016/S0021-9258(18)54518-X
H.S. Wiley, J.J. Herbst, B.J. Walsh, D.A. Lauffenburger, M.G. Rosenfeld, G.N. Gill, The role of tyrosine kinase activity in endocytosis, compartmentation, and down-regulation of the epidermal growth factor receptor. Journal of Biological Chemistry. ,vol. 266, pp. 11083- 11094 ,(1991) , 10.1016/S0021-9258(18)99131-3
F. Brodsky, Living with clathrin: its role in intracellular membrane traffic Science. ,vol. 242, pp. 1396- 1402 ,(1988) , 10.1126/SCIENCE.2904698
C M Stoscheck, G Carpenter, Down regulation of epidermal growth factor receptors: direct demonstration of receptor degradation in human fibroblasts. Journal of Cell Biology. ,vol. 98, pp. 1048- 1053 ,(1984) , 10.1083/JCB.98.3.1048
D T Mahaffey, M S Moore, F M Brodsky, R G Anderson, Coat proteins isolated from clathrin coated vesicles can assemble into coated pits Journal of Cell Biology. ,vol. 108, pp. 1615- 1624 ,(1989) , 10.1083/JCB.108.5.1615
J E Heuser, R G Anderson, Hypertonic media inhibit receptor-mediated endocytosis by blocking clathrin-coated pit formation. Journal of Cell Biology. ,vol. 108, pp. 389- 400 ,(1989) , 10.1083/JCB.108.2.389