Down regulation of epidermal growth factor receptors: direct demonstration of receptor degradation in human fibroblasts.
作者： C M Stoscheck , G Carpenter
关键词:
摘要: The metabolism of the receptor for epidermal growth factor (EGF) has been measured by labeling in vivo with radioactive amino acid precursors and then determining, immunoprecipitation specific anti-EGF antisera, rate degradation when cells are placed a nonradioactive medium. In human fibroblasts EGF (t1/2 = 10.1 h) was faster than total cell protein. When added to medium, half-life prelabeled decreased 1.2 h fibroblasts. These data demonstrate direct analysis protein that during "down regulation" is rapidly degraded. Enhanced observed 5-10 min after addition EGF. EGF-induced blocked methylamine, chloroquine, iodoacetate, or incubation at 25 degrees C. We have also shown down regulation results decrease amount present. quantitated radiolabeling cellular receptor. constitutes approximately 0.0035%
rupress.org
core.ac.uk
jcb.org参考文章(38)
M Ascoli, Internalization and degradation of receptor-bound human choriogonadotropin in Leydig tumor cells. Fate of the hormone subunits. Journal of Biological Chemistry. ,vol. 257, pp. 13306- 13311 ,(1982) , 10.1016/S0021-9258(18)33447-1
Ronald A. Laskey, [45] The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis Nucleic Acids Part I. ,vol. 65, pp. 363- 371 ,(1980) , 10.1016/S0076-6879(80)65047-2
Robert T Schimke, Methods for analysis of enzyme synthesis and degradation in animal tissues. Methods in Enzymology. ,vol. 40, pp. 241- 266 ,(1975) , 10.1016/S0076-6879(75)40020-9
G Carpenter, KJ Lembach, MM Morrison, S Cohen, Characterization of the binding of 125-I-labeled epidermal growth factor to human fibroblasts. Journal of Biological Chemistry. ,vol. 250, pp. 4297- 4304 ,(1975) , 10.1016/S0021-9258(19)41417-8
M N Krupp, D T Connolly, M D Lane, Synthesis, turnover, and down-regulation of epidermal growth factor receptors in human A431 epidermoid carcinoma cells and skin fibroblasts. Journal of Biological Chemistry. ,vol. 257, pp. 11489- 11496 ,(1982) , 10.1016/S0021-9258(18)33787-6
C. Richard Savage, Stanley Cohen, Epidermal Growth Factor and a New Derivative Journal of Biological Chemistry. ,vol. 247, pp. 7609- 7611 ,(1972) , 10.1016/S0021-9258(19)44568-7
S A Buhrow, S Cohen, J V Staros, Affinity labeling of the protein kinase associated with the epidermal growth factor receptor in membrane vesicles from A431 cells. Journal of Biological Chemistry. ,vol. 257, pp. 4019- 4022 ,(1982) , 10.1016/S0021-9258(18)34676-3
L King, S Cohen, G Carpenter, Rapid enhancement of protein phosphorylation in A-431 cell membrane preparations by epidermal growth factor. Journal of Biological Chemistry. ,vol. 254, pp. 4884- 4891 ,(1979) , 10.1016/S0021-9258(17)30094-7
H. Ushiro, S. Cohen, Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes. Journal of Biological Chemistry. ,vol. 255, pp. 8363- 8365 ,(1980) , 10.1016/S0021-9258(18)43497-7
S. Cohen, G. Carpenter, L. King, Epidermal growth factor-receptor-protein kinase interactions. Co-purification of receptor and epidermal growth factor-enhanced phosphorylation activity. Journal of Biological Chemistry. ,vol. 255, pp. 4834- 4842 ,(1980) , 10.1016/S0021-9258(19)85573-4