Fertility of spermatozoa cryopreserved with 2% acetamide or glycerol through artificial insemination in the Japanese white rabbit.
作者： Yasushi OKUDA , Yasunari SEITA , Shin HISAMATSU , Shigenori SONOKI , Masao SHINO
关键词:
摘要: The rabbit is considered to be a valuable laboratory animal. We compared 2% acetamide and glycerol as cryoprotectants in egg-yolk diluent for ejaculated Japanese white spermatozoa improve sperm cryopreservation methods. Fertility through artificial insemination, forward progressive motility plasma membrane integrity of the post-thaw were examined. rates frozen with (27.1 ± 8.3% 24.5 6.5%) significantly (P<0.05) higher than those (16.3 10.9% 14.3 7.6%). Though there was no significant difference kindling rates, litter size females inseminated (6.0 1.1) (3.0 0.4). results indicate that has cryoprotective effect rabbit.
sci-hub.se 参考文章(29)
R H Hammerstedt, P Cramer, P F Watson, E Kunze, A comparison of critical osmolality and hydraulic conductivity and its activation energy in fowl and bull spermatozoa. Journal of Andrology. ,vol. 13, pp. 131- 138 ,(1992) , 10.1002/J.1939-4640.1992.TB01644.X
Naomi NAKAGATA, Tsutomu TAKESHIMA, Cryopreservation of mouse spermatozoa from inbred and F1 hybrid strains Experimental Animals. ,vol. 42, pp. 317- 320 ,(1993) , 10.1538/EXPANIM1978.42.3_317
M. R. Curry, J. D. Millar, P. F. Watson, Calculated optimal cooling rates for ram and human sperm cryopreservation fail to conform with empirical observations. Biology of Reproduction. ,vol. 51, pp. 1014- 1021 ,(1994) , 10.1095/BIOLREPROD51.5.1014
Kenichi Ishibashi, Michio Kuwahara, Yong Gu, Yukio Kageyama, Akira Tohsaka, Fumie Suzuki, Fumiaki Marumo, Sei Sasaki, Cloning and Functional Expression of a New Water Channel Abundantly Expressed in the Testis Permeable to Water, Glycerol, and Urea * Journal of Biological Chemistry. ,vol. 272, pp. 20782- 20786 ,(1997) , 10.1074/JBC.272.33.20782
N. Tada, M. Sato, J. Yamanoi, T. Mizorogi, K. Kasai, S. Ogawa, Cryopreservation of mouse spermatozoa in the presence of raffinose and glycerol Reproduction. ,vol. 89, pp. 511- 516 ,(1990) , 10.1530/JRF.0.0890511
M.R. Curry, B.J. Redding, P.F. Watson, Determination of water permeability coefficient and its activation energy for rabbit spermatozoa. Cryobiology. ,vol. 32, pp. 175- 181 ,(1995) , 10.1006/CRYO.1995.1016
Naomi KASHIWAZAKI, Ena NAKATSUKASA, Akira KATSUMI, Chikashi TACHI, Masao SHINO, Freezing Epididymal Spermatozoa of the Japanese Serow (Capricornis crispus) in Liquid Nitrogen Journal of Reproduction and Development. ,vol. 47, pp. 359- 363 ,(2001) , 10.1262/JRD.47.359
J. A. Gilmore, J. Du, J. Tao, A. T. Peter, J. K. Critser, Osmotic properties of boar spermatozoa and their relevance to cryopreservation Reproduction. ,vol. 107, pp. 87- 95 ,(1996) , 10.1530/JRF.0.1070087
Tokuharu MORIYA, Cryopreservation of semen from Japanese white rabbits for use in teratological studies. Experimental Animals. ,vol. 45, pp. 403- 404 ,(1996) , 10.1538/EXPANIM.45.403
Nobuhiko WATANABE, Hiroshi NAKAGAWA, Shuji KITAJIMA, Enqi LIU, Masatoshi MORIMOTO, Teruo WATANABE, Jianglin FAN, Establishment of a SPF colony of human apo(a) transgenic rabbits by frozen-thawed embryo transfer. Experimental Animals. ,vol. 54, pp. 353- 357 ,(2005) , 10.1538/EXPANIM.54.353