[7] Measurement of translational and rotational diffusion coefficients by laser light scattering

摘要: Publisher Summary This chapter describes the measurement of translational and rotational diffusion coefficients by laser light scattering techniques discusses several applications these to studies in enzymology, including molecular weight determination, absolute size shape enzymes solution, observation conformational changes during protein denaturation. The also problems sample polydispersity multiple scattering. Light-scattering have long provided powerful methods for determination macromolecular weight, size, shape. These rely upon accurate intensity scattered solutions macromolecules. Because lifetimes random fluctuations dielectric constant that produce this are so compared with period incident light, spectrum is far too narrow been measured before recent advent sources, light-mixing spectroscopic techniques, high resolution Fabry-Perot interferometers. has thus referred as “inelastic” or “quasielastic.”