Measurement of the Rotational Diffusion Coefficient of Lysozyme by Depolarized Light Scattering: Configuration of Lysozyme in Solution

摘要: We have determined the rotational diffusion coefficient (DR) of lysozyme from spectrum depolarized light scattered by this protein. The sample was illuminated a single‐frequency He–Ne laser and analyzed using high‐resolution spherical Fabry–Perot interferometer. D20,wR to be (16.7 ± 0.8) × 106/sec at 15% protein concentration. have, in addition, measured translational (DT) employing now‐familiar techniques optical mixing spectroscopy observe polarized find D20,wT (10.6 ± 0.1) × 10−7 cm2/sec, independent concentration between 1% 15%. Our results for DR DT, combined with Perrin's expressions coefficients ellipsoids, indicate that solution is hydrodynamically equivalent prolate ellipsoid revolution major minor axe...