Neuritogenic effect of sea cucumber glucocerebrosides on NGF-induced PC12 cells via activation of the TrkA/CREB/BDNF signalling pathway
作者： Xiaoxu Wang , Peixu Cong , Yanjun Liu , Suyuan Tao , Qinsheng Chen
DOI: 10.1016/J.JFF.2018.04.035
关键词:
摘要: Abstract Sea cucumber glucocerebrosides (SCGs), an important sphingolipid, have extensive functions in inhibiting the growth of cancer cells, but their potential to promote neuronal outgrowth hasn’t been well studied. Herein, we evaluated neuritogenic effects three Cucumaria frondosa SCGs series (SCG-1, SCG-2, and SCG-3) NGF-induced PC12 cells. These exhibited significant a dose-dependent structure-selective manner, which increased ratio neurite-bearing cells expression axonal (GAP-43) synaptic (synaptophysin) proteins. Mechanistic studies suggested that reinforced phosphorylation TrkA, coupled with ERK1/2 activation, then triggered activation CREB, finally resulted marked increase BDNF expression. Furthermore, could be suppressed when accompanied by additions TrkA inhibitor GW441765 or MEK U0126. In conclusion, possess effect promotes signalling downstream from likely via MEK/ERK1/2/CREB/BDNF pathway.
sci-hub.se 参考文章(43)
M. Bothwell, NGF, BDNF, NT3, and NT4. Handbook of experimental pharmacology. ,vol. 220, pp. 3- 15 ,(2014) , 10.1007/978-3-642-45106-5_1
Lei Du, Jie Xu, Yong Xue, Koretaro Takahashi, Chang-Hu Xue, Jing-Feng Wang, Yu-Ming Wang, Cerebrosides from sea cucumber ameliorates cancer-associated cachexia in mice by attenuating adipose atrophy Journal of Functional Foods. ,vol. 17, pp. 352- 363 ,(2015) , 10.1016/J.JFF.2015.05.040
Michael Spedding, Pierre Gressens, Neurotrophins and Cytokines in Neuronal Plasticity Novartis Foundation Symposia. ,vol. 289, pp. 222- 237 ,(2008) , 10.1002/9780470751251.CH18
Hui Xu, Fei Wang, Jingfeng Wang, Jie Xu, Yuming Wang, Changhu Xue, The WNT/β-catenin pathway is involved in the anti-adipogenic activity of cerebrosides from the sea cucumber Cucumaria frondosa. Food & Function. ,vol. 6, pp. 2396- 2404 ,(2015) , 10.1039/C5FO00273G
N. Ichikawa, K. Iwabuchi, H. Kurihara, K. Ishii, T. Kobayashi, T. Sasaki, N. Hattori, Y. Mizuno, K. Hozumi, Y. Yamada, E. Arikawa-Hirasawa, Binding of laminin-1 to monosialoganglioside GM1 in lipid rafts is crucial for neurite outgrowth. Journal of Cell Science. ,vol. 122, pp. 289- 299 ,(2009) , 10.1242/JCS.030338
William R. Mundy, Brian Robinette, Nicholas M. Radio, Theresa M. Freudenrich, Protein biomarkers associated with growth and synaptogenesis in a cell culture model of neuronal development Toxicology. ,vol. 249, pp. 220- 229 ,(2008) , 10.1016/J.TOX.2008.05.012
K Das, Assessment of PC12 cell differentiation and neurite growth: a comparison of morphological and neurochemical measures Neurotoxicology and Teratology. ,vol. 26, pp. 397- 406 ,(2004) , 10.1016/J.NTT.2004.02.006
E. E. Benarroch, Brain-derived neurotrophic factor Regulation, effects, and potential clinical relevance Neurology. ,vol. 84, pp. 1693- 1704 ,(2015) , 10.1212/WNL.0000000000001507
Szu-Ping Chiu, Ming-Jiuan Wu, Pei-Yi Chen, Yi-Ru Ho, Mi-Hsueh Tai, Chi-Tang Ho, Jui-Hung Yen, Neurotrophic action of 5-hydroxylated polymethoxyflavones: 5-demethylnobiletin and gardenin A stimulate neuritogenesis in PC12 cells. Journal of Agricultural and Food Chemistry. ,vol. 61, pp. 9453- 9463 ,(2013) , 10.1021/JF4024678
I. Mocchetti, Exogenous gangliosides, neuronal plasticity and repair, and the neurotrophins Cellular and Molecular Life Sciences. ,vol. 62, pp. 2283- 2294 ,(2005) , 10.1007/S00018-005-5188-Y