Self-regulated glycosylated insulin delivery

摘要: Abstract A self-regulating insulin delivery system, based on the concept of competitive binding between synthetic glycosylated (G-insulin) and glucose to concanavalin (Con A) ligand substrate, has been designed. The two ligands for substrate regulates G-insulin release in relation outside concentration, while a polymeric membrane, serving as peritoneal implant pouch containing Con A, is used control permeability influx efflux. Mono-, di- tri-sugar substituted insulins have characterized. nonimmunogenicity, bioactivity pharmacodynamic activity succinyl amidophenyl glucopyranoside (SAPG-insulin) mannopyranoside (SAPM-insulin) were found be comparable unsubstituted bovine insulin. Initial systems SAPG- or SAPM-insulin with water soluble tetramer contained pouches porous ρ-HEMA cellulose acetate. second system was designed immobilized beads (to prevent leakage) acetate Nucleopore ® membranes. new by crosslinking molecules create gel enclosing Durapore membrane (heat sealable having G-insulins andglucose). fabricated vitro showed short lag time response no leakage molecules. An alternative SAPG-insulin loaded into microcapsules demonstrated due large surface area microcapsules.