Nanotherapeutics Using an HIV-1 Poly A and Transactivator of the HIV-1 LTR-(TAR-) Specific siRNA.
作者： Supriya D. Mahajan , Ravikumar Aalinkeel , Jessica L. Reynolds , Bindukumar Nair , Donald E. Sykes
DOI: 10.4061/2011/719139
关键词:
摘要: HIV-1 replication can be efficiently inhibited by intracellular expression of an siRNA targeting the viral RNA. We used a well-validated (si510) which targets poly A/TAR (transactivator LTR) site and suppresses replication. Nanotechnology holds much potential for impact in field therapeutics, nanoparticles such as quantum rods (QRs) easily functionalized to incorporate forming stable nanoplexes that gene silencing. evaluated efficacy QR-si510 nanoplex suppressing HIV-1-infected monocytic cell line THP-1 measuring p24 antigen levels LTR. Our results suggest is not only effective delivering siRNA, but also longer time period. nanotherapeutics thus enhance systemic bioavailability offer multifunctionality.
hindawi.com参考文章(19)
Supriya D. Mahajan, Indrajit Roy, GaiXia Xu, Ken-Tye Yong, Hong Ding, Ravikumar Aalinkeel, Jessica L. Reynolds, Donald E. Sykes, Bindukumar B. Nair, Elaine Y. Lin, Paras N. Prasad, Stanley A. Schwartz, Enhancing the delivery of anti retroviral drug "Saquinavir" across the blood brain barrier using nanoparticles. Current HIV Research. ,vol. 8, pp. 396- 404 ,(2010) , 10.2174/157016210791330356
Ken-Tye Yong, Jun Qian, Indrajit Roy, Hoon Hi Lee, Earl J. Bergey, Kenneth M. Tramposch, Sailing He, Mark T. Swihart, Anirban Maitra, Paras N. Prasad, Quantum Rod Bioconjugates as Targeted Probes for Confocal and Two-Photon Fluorescence Imaging of Cancer Cells Nano Letters. ,vol. 7, pp. 761- 765 ,(2007) , 10.1021/NL063031M
Gaixia Xu, Ken-Tye Yong, Indrajit Roy, Supriya D. Mahajan, Hong Ding, Stanley A. Schwartz, Paras N. Prasad, Bioconjugated quantum rods as targeted probes for efficient transmigration across an in vitro blood-brain barrier. Bioconjugate Chemistry. ,vol. 19, pp. 1179- 1185 ,(2008) , 10.1021/BC700477U
Jeonghae Park, Peter E. Nadeau, Ayalew Mergia, Activity of TAR in inducible inhibition of HIV replication by foamy virus vector expressing siRNAs under the control of HIV LTR Virus Research. ,vol. 140, pp. 112- 120 ,(2009) , 10.1016/J.VIRUSRES.2008.11.016
S. Hwang, N. Tamilarasu, K. Ryan, I. Huq, S. Richter, W. C. Still, T. M. Rana, Inhibition of gene expression in human cells through small molecule-RNA interactions Proceedings of the National Academy of Sciences of the United States of America. ,vol. 96, pp. 12997- 13002 ,(1999) , 10.1073/PNAS.96.23.12997
Roger J. Pomerantz, RNA interference meets HIV-1: will silence be golden? Nature Medicine. ,vol. 8, pp. 659- 660 ,(2002) , 10.1038/NM0702-659
Sylvie Bannwarth, Anne Gatignol, HIV-1 TAR RNA: the target of molecular interactions between the virus and its host. Current HIV Research. ,vol. 3, pp. 61- 71 ,(2005) , 10.2174/1570162052772924
Jun Qian, Ken-Tye Yong, Indrajit Roy, Tymish Y. Ohulchanskyy, Earl J. Bergey, Hoon Hi Lee, Kenneth M. Tramposch, Sailing He, Anirban Maitra, Paras N. Prasad, Imaging Pancreatic Cancer Using Surface-Functionalized Quantum Dots Journal of Physical Chemistry B. ,vol. 111, pp. 6969- 6972 ,(2007) , 10.1021/JP070620N
Kumiko Ui-Tei, Yuki Naito, Kaoru Saigo, Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi BioMed Research International. ,vol. 2006, pp. 65052- 65052 ,(2006) , 10.1155/JBB/2006/65052
Yamina Bennasser, Man Lung Yeung, Monsef Benkirane, Kuan-Teh Jeang, RNA interference and HIV-1: hits and misses Current Opinion in HIV and AIDS. ,vol. 1, pp. 208- 211 ,(2006) , 10.1097/01.COH.0000221593.49412.56