P43/pro-EMAPII: a potential biomarker for discriminating traumatic versus ischemic brain injury.

摘要: To gain additional insights into the pathogenic cellular and molecular mechanisms underlying different types of brain injury (e.g., trauma versus ischemia), recently attention has focused on discovery study protein biomarkers. In previous studies, using a high-throughput immunoblotting (HTPI) technique, we reported changes in 29 out 998 proteins following acute injuries to rat (penetrating traumatic focal ischemic). Importantly, discovered that one protein, endothelial monocyte-activating polypeptide II precursor (p43/pro-EMAPII), was differentially expressed between these two injury. Among other functions, p43/pro-EMAPII is known pro-inflammatory cytokine involved progression apoptotic cell death. Our current objective verify expression, evaluate potentially important implications differential regulation this development. At multiple time points either penetrating ballistic-like (PBBI), or transient middle cerebral artery occlusion (MCAo) injury, tissue samples (6-72 h), CSF (24 blood h) were collected from rats for analysis. Changes expression assessed by Western blot analysis immunohistochemistry. results indicated significantly increased tissues, CSF, plasma PBBI, but decreased after MCAo compared their respective sham control samples. This may be useful injury-specific biomarker associated with pathologies ischemic provide valuable information directing therapeutics.