Construction of Synthetic Promoter-Based Expression Cassettes for the Production of Cadaverine in Recombinant Corynebacterium glutamicum

作者: Young Hoon Oh , Jae Woo Choi , Eun Young Kim , Bong Keun Song , Ki Jun Jeong

DOI: 10.1007/S12010-015-1701-4

关键词:

摘要: Corynebacterium glutamicum is an important microorganism in the biochemical industry for production of various platform chemicals. However, despite its importance, a limited number studies have been conducted on how to constitute gene expression cassettes engineered C. obtain desired amounts target products. Therefore, this study, six second lysine decarboxylase Escherichia coli, LdcC, were constructed using synthetic promoters with different strengths and examined cadaverine. Among cassettes, E. coli ldcC under PH30 promoter supported highest cadaverine flask fed-batch cultivations. A fermentation recombinant expressing resulted 40.91 g/L 64 h. This report expected contribute toward developing strains features.

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