Animal Model of Granutomatous Entrocolitis Induced by Bacterial Cell Wall Polymers

摘要: Although Crohn’s disease has been postulated to have an infectious etiology based on its gross and microscopic resemblence intestinal tuberculosis yersinia, attempts recover a transmissible agent unsuccessful or poorly resproducible (1). Even in the hands of advocates, recovery rates putative etiologic agents range only from 5–20%. We suggest that toxic, antigenic structural components normal microflora may produce chronic injury susceptible host as alternative explanation conventional concept tissue invasion by replicating pathogenic organism. Purified cell wall polymers derived variety bacterial species are capable initiating sustaining granuloma-tous inflammation animal models. Unique features induced sterile aqueous suspension include spontaneous exacerbations remissions up 12 months after single injection occurring distant site (2). Chronic relapsing erosive arthritis rats intraperitoneal fragments group A streptococci most extensively investigated model (3). The active toxic moiety is peptidoglycan (PG) which resistent biodegradation when convalently bound polysaccharide (PS) glycolipid polymers. PC-PS primary component nearly all found particularly high concentrations Gram positive organisms, thick relative negative bacteria. PG chemically defined structure (Fig. 1) remarkably constant among (4), including enteric flora. PG-PS meet requirements toxicity, immunogenicity, persistence necessary granulomatous (5).