Toward a Biochemical Definition of the Endosomal Compartment
作者： Sandra L. Schmid
DOI: 10.1007/978-1-4615-3026-8_1
关键词:
摘要: The eucaryotic cell is highly compartmentalized, containing many functionally and structurally distinct membrane-bound organelles. Subcellular fractionation a powerful technique being applied to the purification of individual organelles so that they might be studied in biochemical detail. A complete definition vacuolar system involved membrane transport along either endocytic or exocytic pathways requires their both static dynamic terms. An organelle defined terms by its own specific set resident proteins, which give it structural functional identity. temporal location an intracellular traffic route based on kinetics protein transit into through each pathway. To completely define would require homogeneity subsequent characterization. adequately does not necessarily purified but instead rapid, high-yield procedure clearly resolves from other given This review discusses novel method free flow electrophoresis we have preparation endosomal fractions for characterization analytical tracers
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