LncRNA DANCR promotes tumor growth and angiogenesis in ovarian cancer through direct targeting of miR-145.
作者： Xiaojuan Lin , Fan Yang , Xiaorong Qi , Qingli Li , Danqing Wang
DOI: 10.1002/MC.23117
关键词:
摘要: Differentiation antagonizing non-protein coding RNA (DANCR) is a newly identified oncogenic long noncoding found in various cancers. However, the functional role of DANCR tumor angiogenesis and underlying mechanisms are still unclear. The expression was determined ovarian malignant tissues cell lines. revealed by following methods: CD31 staining tissues, matrigel-plug assay HUVEC-related tube formation assay, invasion assay. Enzyme-linked immunosorbent Western blotting, luciferase rescue experiments were used to investigate DANCR-regulating angiogenesis. upregulated cancer cells. Knockdown efficiently impaired growth through inhibition Furthermore, conditional culture medium from DANCR-knockdown cells significantly inhibited HUVEC vitro. Mechanistic investigation indicated that vascular endothelial factor A (VEGF-A, VEGF) plays crucial during cancer. Further results demonstrated miR-145 direct binding target regulation VEGF Collectively, promotional miR-145/VEGF axis. Therefore, may be novel therapy for
sci-hub.se 参考文章(34)
Timothy E. Audas, Stephen Lee, Stressing out over long noncoding RNA. Biochimica et Biophysica Acta. ,vol. 1859, pp. 184- 191 ,(2016) , 10.1016/J.BBAGRM.2015.06.010
Huijuan Wu, ZhengHua Xiao, Ke Wang, Wenxin Liu, Quan Hao, None, MiR-145 is downregulated in human ovarian cancer and modulates cell growth and invasion by targeting p70S6K1 and MUC1 Biochemical and Biophysical Research Communications. ,vol. 441, pp. 693- 700 ,(2013) , 10.1016/J.BBRC.2013.10.053
Chao Zou, Qing Xu, Feng Mao, Dan Li, Chuanxiu Bian, Ling-Zhi Liu, Yue Jiang, Xiaona Chen, Yanting Qi, Xiaolong Zhang, Xuejing Wang, Qiang Sun, Hsiang-Fu Kung, Marie C. Lin, Andreas Dress, Fiona Wardle, Bing-Hua Jiang, Lihui Lai, MiR-145 inhibits tumor angiogenesis and growth by N-RAS and VEGF Cell Cycle. ,vol. 11, pp. 2137- 2145 ,(2012) , 10.4161/CC.20598
Xiaoming Zhang, Ying Dong, Hongjuan Ti, Jing Zhao, Ying Wang, Ting Li, Bo Zhang, Down-regulation of miR-145 and miR-143 might be associated with DNA methyltransferase 3B overexpression and worse prognosis in endometrioid carcinomas Human Pathology. ,vol. 44, pp. 2571- 2580 ,(2013) , 10.1016/J.HUMPATH.2013.07.002
Yu Yin, Zhi-Ping Yan, Na-Na Lu, Qing Xu, Jun He, Xu Qian, Jing Yu, Xin Guan, Bing-Hua Jiang, Ling-Zhi Liu, Downregulation of miR-145 associated with cancer progression and VEGF transcriptional activation by targeting N-RAS and IRS1 Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. ,vol. 1829, pp. 239- 247 ,(2013) , 10.1016/J.BBAGRM.2012.11.006
Zongwei Wang, Charlotta Dabrosin, Xin Yin, Mark M Fuster, Alexandra Arreola, W Kimryn Rathmell, Daniele Generali, Ganji P Nagaraju, Bassel El-Rayes, Domenico Ribatti, Yi Charlie Chen, Kanya Honoki, Hiromasa Fujii, Alexandros G Georgakilas, Somaira Nowsheen, Amedeo Amedei, Elena Niccolai, Amr Amin, S Salman Ashraf, Bill Helferich, Xujuan Yang, Gunjan Guha, Dipita Bhakta, Maria Rosa Ciriolo, Katia Aquilano, Sophie Chen, Dorota Halicka, Sulma I Mohammed, Asfar S Azmi, Alan Bilsland, W Nicol Keith, Lasse D Jensen, None, Broad targeting of angiogenesis for cancer prevention and therapy Seminars in Cancer Biology. ,vol. 35, pp. 00002- 00004 ,(2015) , 10.1016/J.SEMCANCER.2015.01.001
Daniela Luvero, Andrea Milani, Jonathan A. Ledermann, Treatment options in recurrent ovarian cancer: latest evidence and clinical potential Therapeutic Advances in Medical Oncology. ,vol. 6, pp. 229- 239 ,(2014) , 10.1177/1758834014544121
Amrita Ahluwalia, Michael Jones, Tamara Matysiak-Budnik, Andrzej Tarnawski, VEGF and colon cancer growth beyond angiogenesis: does VEGF directly mediate colon cancer growth via a non-angiogenic mechanism? Current Pharmaceutical Design. ,vol. 20, pp. 1041- 1044 ,(2014) , 10.2174/1381612819999131218175905
M. Kretz, D. E. Webster, R. J. Flockhart, C. S. Lee, A. Zehnder, V. Lopez-Pajares, K. Qu, G. X. Y. Zheng, J. Chow, G. E. Kim, J. L. Rinn, H. Y. Chang, Z. Siprashvili, P. A. Khavari, Suppression of progenitor differentiation requires the long noncoding RNA ANCR Genes & Development. ,vol. 26, pp. 338- 343 ,(2012) , 10.1101/GAD.182121.111
Donald T. Yapp, May Q. Wong, Alastair H. Kyle, Shannon M. Valdez, Jenny Tso, Andrew Yung, Piotr Kozlowski, David A. Owen, Andrzej K. Buczkowski, Stephen W. Chung, Charles H. Scudamore, Andrew I. Minchinton, Sylvia S. W. Ng, The differential effects of metronomic gemcitabine and antiangiogenic treatment in patient-derived xenografts of pancreatic cancer: treatment effects on metabolism, vascular function, cell proliferation, and tumor growth Angiogenesis. ,vol. 19, pp. 229- 244 ,(2016) , 10.1007/S10456-016-9503-Z