CD28 Gene Polymorphisms in the Promoter Region Are Associated with Transfusion Reactions: A Functional Study

摘要: Transfusion reactions are mainly induced by the interaction of an antigen and antibody. However, transfusion still occur with implementing crossmatching usage pre-storage leukoreduced blood products. The roles CD28 CTLA4 gene polymorphisms in reaction have been shown, subjects certain single nucleotide (SNPs) or had a significantly higher risk reactions. In total, 40 patients after receiving products were enrolled this study. We focused on SNPs located promoter region (rs1879877, rs3181096, rs3181097, rs3181098) to find out significant SNP. A luciferase reporter assay was used investigate expression level protein affected SNP variation. found that polymorphism rs3181097 associated (p = 0.003 additive model p 0.015 dominant model). Consequently, we investigated biological function (rs1879877 G > T, rs3181096 C A, rs3181098 A) using dual-spectral assay. results showed ex-pression decreased under effect A-allele. This suggested may regulate immune response through decreasing then lead development