Comprehensive molecular analyses of lung adenocarcinoma with regard to the epidermal growth factor receptor, K-ras, MET, and hepatocyte growth factor status.

摘要: Background The mutation and amplification of oncogenic genes are associated with carcinogenesis tumor growth. purpose this study was to clarify the role epidermal growth factor receptor (EGFR), K- ras , MET, hepatocyte (HGF) status in lung adenocarcinoma. Methods Tumor specimens were collected from 183 patients who underwent a complete resection for adenocarcinoma 2003 2007 our department. genetic EGFR investigated by polymerase chain reaction (PCR)-based analyses. Immunohistochemistry real time PCR assays used evaluate MET gene regarding tyrosine phosphorylation amplification, respectively. HGF evaluated immunohistochemistry. Results mutations detected 64 (35%) 17 (9%), 1234/1235 (p-MET 1234/1235) identified 12 (7%) 8 (4%) specimens, Positive expression 104 (57%). An found significantly more frequently females tumors wild type without amplification. A p-MET positive HGF. multivariate survival analysis demonstrated that negative 1234/1235, independently an increased risk poor survival. Conclusions occurrence EGFR/ might be mutually exclusive suggesting several distinct mechanisms development may useful marker predicting prognosis surgical