Rapid Induction of Functional and Morphological Continuity between Severed Ends of Mammalian or Earthworm Myelinated Axons

摘要: The inability to rapidly restore the loss of function that results from severance (cutting or crushing) PNS and CNS axons is a severe clinical problem. As novel strategy help alleviate this problem, we have developed in vitro procedures using Ca2+-free solutions polyethylene glycol (PEG solutions), which within minutes induce functional morphological continuity (PEG-induced fusion) between cut crushed ends myelinated sciatic spinal rats. Using PEG-based hydrogel binds connective tissue provide mechanical strength at lesion site nontoxic nerve tissues earthworms mammals, also vivo permanently maintain earthworm medial giant whose integrity has been restored by PEG-induced fusion after axonal severance. In all these procedures, success measured conduction action potentials through site, presence intact profiles electron micrographs taken and/or intra-axonal diffusion fluorescent dyes across site. These other data suggest application polymeric fusiogens (such as our PEG possibly combined with adherent hydrogels), could lead treatments repair adult including humans.