Isolation of an Antitumor Compound from Agaricus blazei Murill and Its Mechanism of Action

摘要: The Basidiomycete fungus Agaricus blazei Murill has traditionally been used as a health food for the prevention of cancer, diabetes, hyperlipidemia, arteriosclerosis and chronic hepatitis. In present study, we examined antitumor activities various substances isolated from lipid fraction A. blazei. Tumor growth was retarded by oral administration extracted with chloroform/methanol mixture in sarcoma 180-bearing mice. substance activity via silica gel column chromatography, eluted an acetonitrile/methanol (3:2) identified ergosterol direct comparison 1 H NMR mass spectrometry spectral data authentic sample. to mice significantly reduced tumor at doses 400 800 mg/kg administered 20 d without side effects, such decreases body, epididymal adipose tissue, thymus, spleen weights leukocyte numbers induced cancer chemotherapy drugs. Ergosterol had no cytotoxicity against cells. To clarify ergosterol, effects on tumor-induced angiogenesis using two vivo models. Intraperitoneal 5, 10 5 consecutive inhibited neovascularization Lewis lung carcinoma cell-packed chambers, suggesting that either or its metabolites may be involved inhibition neovascularization. Therefore, further inhibitory Matrigel-induced Female C57BL/6 were subcutaneously inoculated Matrigel containing acidic fibroblast factor heparin ergosterol. neovascularization, directly inhibits From these results, it seems likely might due solid tumors. This is first report antiangiogenic substance.