An in vitro approach to assess the toxicity of inhaled tobacco smoke components: Nicotine, cadmium, formaldehyde and urethane

摘要: One of the first lines defence to inhaled toxins is barrier formed by tracheobronchial epithelium, making this ideal region for studying toxicity substances. This study utilises a highly differentiated, three-dimensional, in vitro model human upper respiratory tract epithelium (EpiAirway-100) measure acute toxicological responses well-characterised tobacco smoke components. To determine suitability screening toxicants, EpiAirway tissue (ETM) was treated apically with components (nicotine, formaldehyde, cadmium, urethane) which are known induce variety toxic effects (e.g. cytotoxic, thrombogenic, carcinogenic). A range concentrations were used different mechanisms and severity then compared vivo responses. Similar trends stress response occurred, distinct alterations all four toxins. At high concentrations, cell viability decreased tight junctions degraded, but at sub-toxic epithelial resistance (indicating integrity) increased 20–60% from control. peak coincided an increase secreted protein levels, elevated cytokine release goblet hyperplasia hypertrophy. In conclusion, exposure induces measurable within epithelium. Sub-toxic appear illicit protective increasing mucus secretion mediating immune via release. These comparable responses, indicating potential ETM as tool compounds.